Lea Grinberg, MD, PhD, University of California, San Francisco, CA, discusses the validation of the scRNAseq results for understanding RORB. When using single-cell technology, many computing methods are utilized to acquire results – thus, data with multiple dimensions are simplified for greater understanding. The process of streamlining the data through the use of filters and subjective decision-making allows for the possibility of incurring errors. Therefore, once the data is produced, it is vital to test for validity. Dr Grinberg describes a powerful method of validation, whereby immunohistochemistry is run on postmortem tissues – thereby being able to label proteins that derive from marker genes of neuron vulnerability. Although this is a time-consuming method, it allows for testing in any dimension, identifying markers that could have been introduced by human error. This interview was conducted during the 2022 World Congress on Controversies in Neurology (CONy) meeting.
